Autoimmunity

Introduction: Interferon gamma is one of the most important regulative indices of the body’s immunity system and cellular modifier, in most of the inflammatory diseases such as rheumatoid arthritis, lupus, MS the cytokine balance Th 1/Th2 undergoes broad changes that are related to disease pathogenesis. Quercetin is of fl avonoids family and has a modular function on the immune system. In this research, the eff ect of Quercetin was studied on arthritis pathogenesis due to collagen induction using IFN-γ expression assay and multiplication of T lymphocytes as an immunity cellular index.

 

Materials & Methods: Male Balb/C mice were divided into 3 groups, the fi rst group of BCII and CFA were immunized at the rate of 100 μg at hypodermis of toe and second group on 28th day aft er the fi rst immunization in the tail norm with BCII and IFA and third group on the 42nd day received the second booster, the entire injections were at the rate of 100 μg. Th e arthritis rate due to collagen was measured with wood experiment. Each of the three immunized groups (Prime/Booster1/Booster2) whose toe had infl amed were treated with Quercetin and 14 days aft er the last immunization in each group the rats were subjected to the spinal cord injury, their spleen was extracted and 2x106/cell cellular suspension was prepared in RPMI1640 medium with 10% BCS. IFN-γ measurement and T lymphocyte proliferation rate was evaluated via Elisa, LTT and Brdu method respectively, the results were assessed via One Way ANOVA statistical analysis.

 

Results: The results showed that among the groups treated with Quercetin in each group in the fi rst injection in relation to the control groups a significant diff erence exists at P<0.05 and they had achieved this diff erence to increase IFN-γ expression and cellular proliferation rate with both Booster1 and Booster2 methods in relation to the prime group a signifi cant diff erence was observed at P<0.05.

 

Conclusion: Th e research showed that several types of natural and synthetic flavonoids have an ability to regulate and modify the immune system and establishment of Th 1/Th 2 balance from the pre-infl ammatory cytokines has an inhibitory effect, even quercetin as a fl avonoid can increase the proliferation of T lymphocytes and IFN-γ that is cellular immunity index and can be introduced as a pharmaceutical candidate in the treatment of diseases such as rheumatoid arthritis.