Autoimmunity

Biography

Biography: Xianming Mo

Abstract

We successfully established a 14-color panel to measure circulating immune cells, including granulocytes, basophils, dendritic cells, monocytes, T cells, B cells, NK (natural killer, NK) cells, CD127⁺ILCs and their subsets in one tube of 1ml peripheral blood. Increased frequencies of granulocytes, HLA-DR⁺CD4⁺T cells, CD8⁺ T cells, CD25⁺ DN T cells, HLA-DR⁺DN T cells, HLA-DR⁺DP T cells, CD16^-NK cells, CD16^-CD56^dim NK cells, HLA-DR⁺CD56^dim NK cells, CD11c⁺CD56^dimNK cells, CD25⁺CD56^dimNK cells, HLA-DR⁺ CD56^bright NK cells, CD25⁺ CD56^bright NK cells and CD4⁺CD127⁺ ILCs were observed in patients compared to healthy controls, while decreased frequencies of CD25^hiB cells, T cells, CD4⁺ T cells, T regulatory cells (Tregs), CD25⁺CD8⁺ T cells, CD127⁺CD8⁺ T cells, CD56⁺CD8⁺ T cells, NK cells, CD16⁺NK cells, CD16^bright CD56^dim NK cells and CD127⁺ ILCs were observed in patients compared to healthy controls. The frequencies of B cells, CD25⁺CD56^bright NK cells, CD16⁺ NK cells and CD16^- NK cells were correlated with disease activity. There were significance differences in frequencies of B cells, CD127⁺CD8⁺T cells, basophils, DCs, HLA-DR⁺ CD56^bright NK cells, intermediate monocytes, CD25⁺ CD56^dim NK cells, CD16⁺ CD4⁺ T cells, CD25⁺ CD56^bright NK cells, CD11c⁺ CD56^bright NK cells, HLA-DR⁺ CD4⁺ T cells, HLA-DR⁺ Tregs, CD16⁺ CD8⁺ T cells, CD25⁺ CD8⁺ T cells, CD11c⁺ CD4⁺ T cells, CD25⁺ DP T cells and CD16^- CD56^bright NK cells between autoantibodies positive groups and autoantibodies negative groups. We observed diverse alterations in the peripheral immune cells in SLE patients and some cell subsets were related with SLE clinical variables. The immune cells may take part in the development of SLE disease.