2^nd International Conference on Autoimmunity November 06-07, 2017 Frankfurt, Germany

Ioannis Gkougkourelas, is expertise in Hematology, Internal Medicine (General Medicine), Clinical Immunology in Aristotle University of Thessaloniki, Greece. He has many research publications & attended national & international conferences

Introduction: Lupus nephritis is an ominous manifestation of Systemic Lupus Erythematosus (SLE) requiring aggressive immunosupressants. Biopsy is the gold standard for diagnosis. A lot of studies have investigated the possible role of various biomarkers in blood and/or urine to aiming to early discovery of kidney involvement in SLE with contradictory results. No single biomarker has been established as a screening tool for the Lupus nephritis. Soluble Triggering Receptor Expressed on Myelocytes (sTREM-1) has been correlated with activity score of SLE (SLEDAI) but few studies have been investigated sTREM-1 in Lupus nephritis Patients and methods: We investigated the levels of sTREM-1 in urine of 8 SLE patients with biopsy proven nephritis and 10 patients with active SLE but no kidney involvement ( according to EULAR criteria). Th e patients were choosen randomly by the Outpatient clinic of Clinical Immunology Unit of 2nd Internal Medicine Dpt. Th e overall severity of the disease in both groups was at the same class according the SLEDAI index. We performed enzyme linked immune sorbent assay in the urine by the commercially available ki (USCN Life Sciences) following the manufactures protocol. Results were expressed as mean+/- sd. Mann –Whitney test was performed to assess possible signifi cant diff erences, p<0.05 was considered signifi cant. Results: Th e mean value of sTREM-1 in patients with nephritis was 28+/-2.3 pg/ml and the mean SLEDAI index was 13+/-5. Patients with SLE without nephritis (mean SLEDAI 12+/-4) was signifi cantly lower 6+/-5 pg/ml. Discussion: sTREM-1 is increased in urine of patients with Lupus Nephritis relative to SLE patients without kidney involvement. sTREM-1 could be a candidate biomarker measured in urine helping the physician to discriminate patients suspicious for SLE nephritis.

Jae Chul Lee, Department of Biology, School of Life Sciences, Chungbuk National University, Cheongju, Korea & has many research publications & attended national & international conferences

Osteoarthritis (OA) is a degenerative joint disease characterized by abrasion, and ultimately, destruction of the articular cartilage and trabecular bone loss. OA is still considered a devastating disease, which requires an aggressive therapeutic approach. Despite the therapeutic potential of human adipose-derived mesenchymal stem cells (AD-MSCs), the molecular parameters needed to defi ne the stemness remain largely unknown. Using high-density oligonucleotide microarrays, the diff erential gene expression profi les between a fraction of human adipose-derived (AD) mononuclear cells and its MSC subpopulation were obtained. Of interest, a subset of 58 genes preferentially expressed at 7-fold or higher in the group treated with human AD-MSCs. Th is subset contained numerous genes involved in the infl ammatory response, immune response, lipid metabolism, cell death, cell proliferation, and DNA repair. Additionally, four protein networks were constructed. Th e interaction network consisted of 46 proteins encoded by up-regulated genes. However, the interaction network also consisted of 38 proteins encoded by down-regulated genes. My results provide a basis for a more reproducible and reliable quality control using genotypic analysis for the defi nition of human AD-MSCs. Th erefore, these results will provide a basis for studies on molecular mechanisms controlling the core properties of human MSCs.

Farida Karim has completed her MBBS from Jinnah Medical and Dental College Karachi and internship from Aga Khan University Hospital. She is working currently as Medical Offi cer in the Aga Khan University Hospital, Pakistan which is the best and most advanced healthcare service organization. She has published three artilces and working on several other projects that are under reviewing process.

Objective: To determine the clinical and immunological characteristics and short-term outcome of children with systemic lupus erythematosus (cSLE) presented at a tertiary care center in Karachi, Pakistan. Design: A descriptive observational study conducted at the Paediatric Rheumatology Clinic of Aga Khan University Hospital (AKUH), Karachi, from January 2011 to April 2015. Methodology: Data of children <16 years of age, admitted to the Paediatric ward, diagnosed with cSLE, was studied. Results: 32 children satisfying the criteria of American College of Rheumatology (ACR) for cSLE were enrolled. A female predominance was observed, with 87.5% of the patients being female. Mean age at symptom onset was 10.5+2.7 years and 8.8+2.1 years in females and males respectively. Mean age at diagnosis was 11.3+2.8 years in females and 9.4+1.9 years in males. Fever was the most common non-specifi c symptom found in 84% patients. 69% children were found to be anemic and 56% had signs of arthritis at presentation. Renal involvement was observed in 47% patients. Th e most common immunological markers were found to be serum Anti-neutrophil antibodies (ANA), positive in 88% patients, followed by Anti-double-stranded DNA antibodies (anti ds-DNA), raised in 81% cases. Overall response rate to therapy was 50% in 20 children who were followed for four years. Conclusion: We found that cSLE encompasses a wide variety of manifestations with a female preponderance. Fever and Arthralgia are the most frequent clinical fi ndings. Hemolytic anemia is the most common laboratory abnormality, with ANA and Anti ds-DNA positivity in a majority of patients.

Prof. Dr. Hedef El-Yassin was pursuing as faculty in University of Baghdad in the Department of Biochemistry and in College of Medicine. He has much publication and in reputed journals attended in National conferences

Background & Aim: Older age is usually accompanied by functional decline due to loss of skeletal muscle mass and quality. Sarcopenia and muscle frailty are both highly relevant entities with regards to functionality and autonomy of older adults. European Working Group on Sarcopenia in Older People (EWGSOP) founded in 2009 has put the main criteria for clinical diagnosis of sarcopenia including the following domains: Physical performance, Muscle strength and /or Muscle mass. Sarcopenia is also associated with modifications in biological functions, including inflammation, glucose, regulation, hormone production, cellular, communication and protein storage. However, no laboratory guidelines' have yet been established for confirmatory testes of the diagnosis. The aim of the present work is to shed a light on the variations of some inflammatory markers in a group of sarcopenic Iraqi patients and to aid in the clinical diagnosis of the disease. Subjects & Methods: The study included (100) sarcopenic subjects (50 male and 50 female) and (50) non sarcopenic subjects (25 male and 25 female). Information were taken from each subject (age, gender, patients with inflammatory disease (rheumatoid arthritis systemic lupus erythematosus (SLE)), diabetes mellitus, thyroid disease and patients taking steroid therapy were excluded. Subjects with primary sarcopenia were diagnosed by: Short Physical Performance Battery (SPPB) and dual-energy X-ray absorptiometry (DEXA) to determine (Appendicular skeletal muscle mass (ASM), Total lean body mass (TLBM) and Body Mass Index (BMI)). There are two methods used in the study process, 1-Clinical diagnostic measurements: Physical performance: Short Physical Performance Battery (SPPB), Muscle strength: hand grip. 2-Biological markers (in serum): Markers of inflammation: interleukin (IL)-6, alpha1-antichymotrypsin (ACT). Results: Mean values of (α1ACA) in control group were more than study group and men more than women and their mean values were decreasing with aging. While (IL-6,) mean values in study group, were more than control group and in women more than men except BMI in male more than female and values increase with increasing age. Conclusions: Mean values of (α1ACA) in control group were more than study group and in women less than men because sarcopenia is defined as a reduction in ASM/height2, and total lean body. Alpha 1-antichymotrypsin has a direct relation with ASM. While (IL-6,) have indirect relation with ASM and α1ACA. So, clinical variables values were increased: with age, in study group more than control group in women more than men.